Buying and applying Mycorrhizal Fungi

Written by Donald Lester.

There are many species of fungi, bacteria, viruses and protozoa commercially available for the biological control of insects, diseases and weeds. Some products offer mixtures of various organisms. This article will cover one particularly easy-to-use type of fungi that help control soil diseases and makes otherwise unavailable soil nutrients available to plants – this fungi is known as mycorrhizae.

Mycorrhizal fungi are a group of fungi that live on and within plant roots in a symbiotic or mutually beneficial relationship.The fungus attaches itself to the plant root and then grows out into the soil with very fine “roots” or mycelium.These mycorrhizal root systems increase the absorbing area of plant
roots 10 to 1,000 times, thus greatly improving the ability of plants to utilize soil resources. In return, the fungus receives the finished product in the form of carbohydrates, proteins and sugars. Mycorrhizal fungi are able to absorb all of the 15 major macro and micro nutrients required for plant growth. Mycorrhizal fungi release powerful chemicals into the soil that dissolve hard-to-capture nutrients such as phosphorus. It does this so well that mycorrhizae do not perform well in phosphorus-rich soils.This phenomenon is particularly important in plant nutrition and explains why non-mycorrhizal plants require higher levels of fertility to maintain their health.

Moreover, this extended root system is very important for water uptake and storage. In non-irrigated conditions, plants inoculated with mycorrhizae are under far less drought stress compared to untreated plants.

Over 95 per cent of crops not only respond positively to colonization by mycorrhizal fungi, but they may suffer in the absence of mycorrhizae (Kendra Baumgartner, USDA California). Mycorrhizal fungi in nature are lost due to soil disturbances such as mining, construction or erosion; strong acid or chemical treatments; pesticides, excessive heat, drought or flooding; and denial of oxygen or water by asphalt, concrete, soil compaction and roads. In indoor gardens, mycorrhizae must be introduced into sterilized soils and maintained because of losses due to the use of strong sterilizers, waterlogged soils low in oxygen and excessive fertilization. Mycorrhizae work well within integrated pest management (IPM) programs and organic programs; are effective in resistance management; break down crop residue, thus freeing up valuable nutrients for plants; and are non-burning and non-phytotoxic to plants.

There are two main types of mycorrhizae: ecto and endo.

Endomycorrhizal fungi, also known as AM (arbuscular mycorrhizal):

This group of mycorrhizal fungi is important in non-woody plants, including many agricultural crops, grasses and turf. They are sometimes called vesicular-arbuscular mycorrhizae (VAM) due to the specialized structures that the fungus creates. Although this type of mycorrhizae invades plant roots, it does not harm the plant.

Ectomycorrhizal fungi:

This group of mycorrhizal fungi grows between root cells and forms a sheath or mantle around plant roots, but it does not actually invade the plant cells.They are important to many woody perennials, bushes, shrubs and trees.

There is great potential for the use of mycorrhizal fungi as inoculants especially in the production and protection of high-value greenhouse-grown crops or transplanted vegetable crops such as tomatoes and other bedding plants. If using mycorrhizae as a control rather than preventative, it is best to treat infections early. If mycorrhizae are applied too late, then the results will be disappointing. Mycorrhizae are not a miracle control, but with proper usage and realistic expectations, you can have Mother Nature working for you.

Below are some general guidelines for using mycorrhizae in various greenhouse and hydroponic environments.As with any product, strict adherence to the label instructions is necessary for optimum performance.


Aeroponic systems are a more highly aerated environment than traditional hydroponics and have proven to be an excellent system for growing mycorrhizae without a physical substrate. This system allows the grower to actually see the fungi growing on root surfaces. Liquid formulations of mycorrhizae can be added to the nutrient solution and delivered through the misting system directly to the roots. Some filtering of the liquid before application may be required to prevent nozzle fouling.

Transplanting into Containers

Apply granules or liquid formulation directly into the planting hole, making sure that the root ball has good contact with the mycorrhizae. Some products come in convenient biodegradable teabags or sachets, as they are known in Europe, that can be dropped into the planting hole, making application quick and easy.As the plants grow, maintenance applications can be applied as soil drenches. Bulk granular formulations can be placed in a sock or similar material and soaked for several hours to release the organisms and activate them.This liquid can then be used as a soil drench for maintenance applications as the season progresses.

Mix with growing Media

Many commercial potting mixes already contain mycorrhizal fungi incorporated into them. However, bulk granules of mycorrhizae can be mixed in with the soil before transplanting or applied to the soil surface after transplanting, much like a granular fertilizer. The incorporation of mycorrhizal fungi is especially important in sterilized soils to regain a population of beneficial micro-organisms to out-compete pathogens. Bark, calcined clay, expanded clay and perlite provide good aeration. Peat and vermiculite hold more water and allow air to penetrate better than sand. Mycorrhizae are aerobic, meaning that they require oxygen.They can grow in anaerobic or reduced oxygen environments like rice, but their populations are greatly reduced in these environments. So it is best not to waterlog the media for too long.

Bare root

Bare root plants can be dipped into solutions containing mycorrhizal fungi that have been pre-activated. Mycorrhizal fungi are compatible with most common commercial rooting powders and water-absorbent gels.The teabag formulations can be soaked in water just like a regular teabag, and the solution can be used for dipping. Granules can also be soaked in water for a few hours, with the water being used as a base for a dipping solution as well.

Starter Cubes

There are basically four kinds of starter cubes: rock wool, oasis cubes, organo-cubes and peat pots.The most favored types are rockwool, organo-cubes and oasis cubes as they retain water well and also hold enough air so that the medium does not stay too wet.

Preparing the starter cubes varies with the kind of starter cube you are using. Organo-Cubes come ready for use.The oasis and peat pot cubes should be pre-soaked with water that is adjusted to pH 6.0 – 6.5.The cubes need to be saturated and then allowed to drain.The cubes are now ready to use. Rockwool has a high pH and, as a result, must be treated differently. Rockwool cubes must be pre-soaked with water that is pH adjusted to 5.0. This low pH acid solution will balance the overall pH to a level that plants require.The cubes should be soaked for about 24 hours and then drained.They are then ready for use.

Once the starter cubes are prepared, you can plant the seed and apply mycorrhizal fungi. Gently cover the seed by packing some of the seed cube material from around the hole over the seed.

Sand Culture

Mycorrhizae, especially Glomus sp., do very well in sand culture systems through which recycled nutrient solution is passed several times a day (Ojala and Jarrell, 1980). Simply inoculate the nutrient reservoir with mycorrhizae according to the label. Be careful in the use of strong compost teas or other green manure materials as food sources for mycorrhizae because the low pH of these materials can adversely affect mycorrhizae growth. Similarly, the use of molasses and other sugars as a mycorrhizal food source can stimulate pathogen development. Using humic acid as a carbon food source will help the mycorrhizae, and the soil-binding nature of humic acid will help build structure in the sand.

Sawdust Bags

Mycorrhizae can be incorporated into sawdust bags.The bag should be presoaked with nutrient solution 48 hours prior to setting the plants out
in the bags.Transplant as usual with mycorrhizae pre-mixed in the sawdust or with mycorrhizae placed in the immediate vicinity of the root zone.


Seeds can be put in direct contact with mycorrhizae at planting time. This ensures that the young roots are colonized early on for protection against water mould root rots like Pythium and Phytophthora. However, be sure that the mycorrhizae are compatible with any fungicides that may be applied with the seed or used as a seed treatment.

Mycorrhizae need carbon as a food source. Humic acids are a concentrated organic matter and are often used to supplement mycorrhizae in soil systems. Do not use dry dog food or other carbon sources not recommended by the manufacturer. Some growers try to supplement mycorrhizae populations with sugars and other materials that can promote pathogen development and lead to disastrous results.

Follow-up applications to the surface of the medium every few months will help maintain a healthy population of these beneficial fungi. In fact, if the roots have between 40 to 80 per cent coverage with white mycorrhizae fungi, then you have a healthy population protecting your plants.

Shopping for Mycorrhizal Fungi

There are a lot of mycorrhizal products on the market today. Growers are often wary of new products that suddenly appear on the market as copy-cats of more well-known products. Here are some features and specifications to look for when buying mycorrhizae products.

First, look for stable formulations. Over the years, the packaging and use of beneficial micro-organisms have become easier to use and more convenient. Many products now come as powders or liquids. One mycorrhizal product comes in a biodegradable “teabag” of powder and is often referred to as a teabag in the U.S. or a sachet in Europe.This powder contains spores and other fungal structures that need moisture to come out of dormancy. Dry formulations of mycorrhizae have a typical shelf life of 16-18 months, although two years is not uncommon. Do not buy products that require refrigeration or special handling. Refrigerated products may have been allowed to get warm and be reduced in effectiveness long before you receive them. Stable products come in liquid, granular or teabag formulations.

Second, look for combinations of mycorrhizae species in one product.
A mixture will often give protection against more pathogens than a single mycorrhizal species. Also, several species of mycorrhizae may be combined to give longer season control because where one species starts to lose effectiveness, the other may extend the protection. Moreover, products with a mixture of ectomycorrhizae and endomycorrhizal are applicable to a wider range of crops than a product with only one or the other. Keep in mind that beets, broccoli and canola do not respond to mycorrhizal.

Third, look for a guaranteed analysis on the label. These numbers will give you the number of spores or colony-forming units (CFUs) in the product. The higher the number then the more fungal “seeds” you have to inoculate your plant roots.

Fourth, shelf life is important. It does you no good to have a large number of spores at the time of manufacture only to have nothing but water to apply when you are ready to use the product.A long shelf life ensures the product is live and active when you need it.

Fifth, do your homework and see how long the product has been on the market. Reputable companies have been selling the product for many years and have a track record. Companies selling poor performing products will not be in the market for very long.

Sixth, look for products that operate over a wide range of temperatures. Mycorrhizal fungi, like any living organism, slow down or die in extreme temperatures.The temperature range in which the product is effective gives you an indication of how hardy the mycorrhizal strains are. Often manufacturers will mix mycorrhizae that have different points of inactivation due to high or low temperatures, again to extend protection time.

Lastly, make sure the mycorrhizal product is organically certified.This ensures that the product does not contain any harmful human pathogens, genetically modified organisms or synthetically produced ingredients.

Incorporating mycorrhizae into your indoor garden or growing operation is easy, safe and environmentally friendly. Look for mycorrhizal fungi products in your gardening catalogue, local nursery or garden centre. By using mycorrhizae, you will be promoting a more balanced population of soil micro-organisms and reduce your use of harsh, toxic chemicals. And you will save yourself costly and worrisome disposal problems associated with chemical alternatives. Now that you’ve learned a little about mycorrhizal, perhaps you will feel more confident in buying and using mycorrhizal fungi in your indoor garden or protected cropping system.

High Quality Mycorrhizal ProductLow Quality Mycorrhizal Product
Liquid, gradular, or teabag formulationRefrigerated product
Mixture of ecto and endo typesSingle species product
High CFU or spore countLow CFU or spore count
Long shelf life – two yearsShort shelf life
Reputable manufacturerQuestionable source
Active over wide temperature rangeActive over narrow temperature range
Organic certificationNot organically certified

To read the original paper, click here.

Leave a Comment

Your email address will not be published. Required fields are marked *